HuH-7-CAS9 cell line

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

HuH-7-CAS9 Order

Catalog#

YC-D001-Cas9-H

Size

1*10^6

Transfection Culture Medium

Transfection Culture Medium(HuH-7)

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

HuH-7-CAS9

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:3

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the HuH-7 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HuH-7-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
HuH-7-CAS9	Cas9	20.01689911	3.60826302
HuH-7-CAS9	h-actin-B	16.40863609	3.60826302
HuH-7	Cas9	35.83909988	18.00432014
HuH-7	h-actin-B	17.83477974	18.00432014

Cutting Efficiency Validation

HuH-7-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the HuH-7-CAS9 stable cell pool which is electroporated by NONO gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HuH-7			STR Info (Cell bank) Cell Line:HuH-7
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	10	11		10	11
D7S820	11	11		11	11
D16S539	10	10		10	10
VWA	16	18		16	18
TH01	7	7		7	7
AMEL	X	X		X	X
TPOX	8	11		8	11
CSF1PO	11	11		11	11

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

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